A Mutasynthetic Library of Platensimycin and Platencin Analogues

Org Lett. 2016 Sep 16;18(18):4606-9. doi: 10.1021/acs.orglett.6b02248. Epub 2016 Sep 6.

Abstract

Inactivation of ptmB1, ptmB2, ptmT2, or ptmC in Streptomyces platensis SB12029, a platensimycin (PTM) and platencin (PTN) overproducer, revealed that PTM and PTN biosynthesis features two distinct moieties that are individually constructed and convergently coupled to afford PTM and PTN. A focused library of PTM and PTN analogues was generated by mutasynthesis in the ΔptmB1 mutant S. platensis SB12032. Of the 34 aryl variants tested, 18 were incorporated with high titers.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adamantane / chemical synthesis*
  • Adamantane / chemistry
  • Aminobenzoates / chemical synthesis*
  • Aminobenzoates / chemistry
  • Aminophenols / chemical synthesis*
  • Aminophenols / chemistry
  • Anilides / chemical synthesis*
  • Anilides / chemistry
  • Molecular Structure
  • Polycyclic Compounds / chemical synthesis*
  • Polycyclic Compounds / chemistry
  • Small Molecule Libraries / chemical synthesis*
  • Small Molecule Libraries / chemistry
  • Stereoisomerism

Substances

  • Aminobenzoates
  • Aminophenols
  • Anilides
  • Polycyclic Compounds
  • Small Molecule Libraries
  • Adamantane
  • platensimycin
  • platencin