Dual-Stage Crosslinking of a Gel-Phase Bioink Improves Cell Viability and Homogeneity for 3D Bioprinting

Karen Dubbin; Yuki Hori; Kazuomori K Lewis; Sarah C Heilshorn

doi:10.1002/adhm.201600636

Dual-Stage Crosslinking of a Gel-Phase Bioink Improves Cell Viability and Homogeneity for 3D Bioprinting

Adv Healthc Mater. 2016 Oct;5(19):2488-2492. doi: 10.1002/adhm.201600636. Epub 2016 Sep 1.

Authors

Karen Dubbin¹, Yuki Hori¹, Kazuomori K Lewis², Sarah C Heilshorn³

Affiliations

¹ Materials Science and Engineering, Stanford University, Stanford, CA, 94305, USA.
² Chemical Engineering, Stanford University, Stanford, CA, 94305, USA.
³ Materials Science and Engineering, 476 Lomita Mall, Stanford University, Stanford, CA, 94305, USA. Heilshorn@stanford.edu.

PMID: 27581767
DOI: 10.1002/adhm.201600636

Abstract

Current bioinks for cell-based 3D bioprinting are not suitable for technology scale-up due to the challenges of cell sedimentation, cell membrane damage, and cell dehydration. A novel bioink hydrogel is presented with dual-stage crosslinking specifically designed to overcome these three major hurdles. This bioink enables the direct patterning of highly viable, multicell type constructs with long-term spatial fidelity.

Keywords: bioink; biomaterials; bioprinting; hydrogels; tissue engineering.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Bioprinting / methods*
Cell Line
Cell Survival / drug effects*
Hydrogel, Polyethylene Glycol Dimethacrylate / pharmacology*
Mice
NIH 3T3 Cells
Printing, Three-Dimensional
Tissue Engineering / methods*
Tissue Scaffolds

Substances

Hydrogel, Polyethylene Glycol Dimethacrylate

Abstract

Publication types

MeSH terms

Substances

Grants and funding