Spatiotemporal Fluctuation Analysis: A Powerful Tool for the Future Nanoscopy of Molecular Processes

Carmine Di Rienzo; Enrico Gratton; Fabio Beltram; Francesco Cardarelli

doi:10.1016/j.bpj.2016.07.015

Spatiotemporal Fluctuation Analysis: A Powerful Tool for the Future Nanoscopy of Molecular Processes

Biophys J. 2016 Aug 23;111(4):679-685. doi: 10.1016/j.bpj.2016.07.015.

Authors

Carmine Di Rienzo¹, Enrico Gratton², Fabio Beltram¹, Francesco Cardarelli³

Affiliations

¹ NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, Pisa, Italy; Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, Pisa, Italy.
² Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California.
³ Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, Pisa, Italy. Electronic address: francesco.cardarelli@iit.it.

Abstract

The enormous wealth of information available today from optical microscopy measurements on living samples is often underexploited. We argue that spatiotemporal analysis of fluorescence fluctuations using multiple detection channels can enhance the performance of current nanoscopy methods and provide further insight into dynamic molecular processes of high biological relevance.

MeSH terms

Image Processing, Computer-Assisted*
Microscopy, Fluorescence / methods*
Nanotechnology / methods*
Spatio-Temporal Analysis