Data on the concentrations of etoposide, PSC833, BAPTA-AM, and cycloheximide that do not compromise the vitality of mature mouse oocytes, parthenogencially activated and fertilized embryos

Jacinta H Martin; Elizabeth G Bromfield; R John Aitken; Tessa Lord; Brett Nixon

doi:10.1016/j.dib.2016.07.046

Data on the concentrations of etoposide, PSC833, BAPTA-AM, and cycloheximide that do not compromise the vitality of mature mouse oocytes, parthenogencially activated and fertilized embryos

Data Brief. 2016 Jul 30:8:1215-20. doi: 10.1016/j.dib.2016.07.046. eCollection 2016 Sep.

Authors

Jacinta H Martin¹, Elizabeth G Bromfield¹, R John Aitken¹, Tessa Lord¹, Brett Nixon¹

Affiliation

¹ Priority Research Centre for Reproductive Science, Discipline of Biological Sciences and Hunter Medical Research Institute, University of Newcastle, Callaghan, NSW 2308, Australia.

Abstract

These data document the vitality of mature mouse oocytes (Metaphase II (MII)) and early stage embryos (zygotes) following exposure to the genotoxic chemotherapeutic agent, etoposide, in combination with PSC833, a selective inhibitor of permeability glycoprotein. They also illustrate the vitality of parthenogencially activated and fertilized embryos following incubation with the calcium chelator BAPTA-AM (1,2-Bis(2-aminophenoxy)ethane- N,N,N',N'-tetraacetic acid tetrakis (acetoxymethyl ester)), cycloheximide (an antibiotic that is capable of inhibiting protein synthesis), and hydrogen peroxide (a potent reactive oxygen species). Finally, they present evidence that permeability glycoprotein is not represented in the proteome of mouse spermatozoa. Our interpretation and discussion of these data feature in the article "Identification of a key role for permeability glycoprotein in enhancing the cellular defense mechanisms of fertilized oocytes" (Martin et al., in press) [1].

Keywords: BAPTA-AM; Cyloheximide; Embryo; Etoposide; Oocytes; PSC833; Parthenote; Vitality.