Aim: To demonstrate the role of RpoE during the later stage of hyperosmotic stress in Salmonella.
Materials & methods: Expressions of SPI-1 and SPI-2 under hyperosmotic stress for 120 min were investigated by a microarray, and the invasion and intracellular survival of wild-type and ΔrpoE strains were compared. The global differential expression of bacterial proteins between the wild-type and ΔrpoE strains was examined after 120 min of hyperosmotic stress.
Results: SPI-1 and SPI-2 were repressed, and the invasion and intracellular survival were defected in the ΔrpoE strain. Thirteen bacterial-associated proteins and 11 secreted proteins differed significantly between the wild-type and ΔrpoE strains.
Conclusion: RpoE may promote invasion and intracellular survival by regulating the expression of SPI-1 and SPI-2.
Keywords: RpoE; SPI-1; SPI-2; Salmonella enterica serovar Typhi; SipC; hyperosmotic stress.