Epitope analysis of DQ6-reactive antibodies in sera from a DQ6-positive transplant candidate sensitized during pregnancy

Transpl Immunol. 2016 Sep:38:15-8. doi: 10.1016/j.trim.2016.07.004. Epub 2016 Jul 26.

Abstract

This case report describes DQ6-reactive serum antibody reactivity in a patient who types as DQ6. DNA typing showed DQB1*06:09 on the antibody producer and serum reactivity with DQB1*06:01, *06:02 and *06:03 but not with *06:04 and *06:09. HLAMatchmaker serum analysis showed antibody reactivity with a new antibody-verified 85VA eplet on DQB but additional reactivity with DQB1*02:01 could not be readily interpreted. After applying the nonself-self algorithm of HLA immunogenicity we have identified a new DQB epitope structurally described as 140A2+130R+135D and shared by DQB1*02:01 and DQB1*05:01 and DQB1*06:02 of the immunizer.

Keywords: Epitope; HLA; HLA antibodies; HLAMatchmaker; Nonself-self.

Publication types

  • Case Reports

MeSH terms

  • Algorithms
  • Alleles
  • Autoantigens / genetics
  • Autoantigens / immunology
  • Autoantigens / metabolism*
  • Epitope Mapping / methods*
  • Female
  • HLA-DQ Antigens / genetics
  • HLA-DQ Antigens / immunology
  • HLA-DQ Antigens / metabolism*
  • High-Throughput Nucleotide Sequencing
  • Histocompatibility Testing
  • Humans
  • Immunization*
  • Isoantibodies / blood
  • Isoantigens / genetics
  • Isoantigens / immunology
  • Isoantigens / metabolism*
  • Middle Aged
  • Pregnancy
  • Protein Conformation
  • Registries
  • Transplant Recipients
  • Waiting Lists

Substances

  • Autoantigens
  • HLA-DQ Antigens
  • HLA-DQ6 antigen
  • Isoantibodies
  • Isoantigens