Utilizing on- and off-line monitoring tools to follow a kinetic resolution step during flow synthesis

Magn Reson Chem. 2017 Apr;55(4):348-354. doi: 10.1002/mrc.4494. Epub 2016 Aug 11.

Abstract

In situ reaction monitoring tools offer the ability to track the progress of a synthetic reaction in real time to facilitate reaction optimization and provide kinetic/mechanistic insight. Herein, we report the utilization of flow NMR, flow IR, and other off-line spectroscopy tools to monitor the progress of a flow chemistry reaction. The on-line and off-line tools were selected to facilitate the stereoselective kinetic resolution of a key racemic monomer, which lacked a chromophore, making conventional reaction monitoring difficult. Copyright © 2016 John Wiley & Sons, Ltd.

Keywords: 1H NMR; enzyme; flow IR; flow NMR; flow synthesis; kinetic resolution; reaction monitoring.