Fluorescent vinblastine probes for live cell imaging

Chem Commun (Camb). 2016 Aug 2;52(64):9953-6. doi: 10.1039/c6cc04129a.

Abstract

Herein we describe the synthesis of several fluorescent analogues of the clinically approved microtubule destabilizing agent vinblastine. The evaluated probes are the most potent described and provides the first example of uptake, distribution and live cell imaging using this well known antimitotic agent.

MeSH terms

  • Cell Line, Tumor
  • Cell Survival / drug effects
  • Cell Survival / physiology
  • Dose-Response Relationship, Drug
  • Fluorescent Dyes / analysis*
  • Fluorescent Dyes / metabolism
  • Fluorescent Dyes / pharmacology
  • Humans
  • Microscopy, Fluorescence / methods
  • Protein Structure, Secondary
  • Tubulin / chemistry
  • Tubulin / metabolism
  • Vinblastine / analysis*
  • Vinblastine / metabolism
  • Vinblastine / pharmacology

Substances

  • Fluorescent Dyes
  • Tubulin
  • Vinblastine