Applications of a Rapid and Sensitive Dengue DUO Rapid Immunochromatographic Test Kit as a Diagnostic Strategy during a Dengue Type 2 Epidemic in an Urban City

Hsin-I Shih; Hsiang-Chin Hsu; Chi-Jung Wu; Chih-Hao Lin; Chia-Ming Chang; Yi-Fang Tu; Chih-Chia Hsieh; Chih-Hsien Chi; Tzu-Ching Sung

doi:10.1371/journal.pone.0158437

Applications of a Rapid and Sensitive Dengue DUO Rapid Immunochromatographic Test Kit as a Diagnostic Strategy during a Dengue Type 2 Epidemic in an Urban City

PLoS One. 2016 Jul 14;11(7):e0158437. doi: 10.1371/journal.pone.0158437. eCollection 2016.

Authors

Hsin-I Shih^{1

2

3

4}, Hsiang-Chin Hsu¹, Chi-Jung Wu^{5

4}, Chih-Hao Lin^{1

2}, Chia-Ming Chang^{6

7}, Yi-Fang Tu⁸, Chih-Chia Hsieh¹, Chih-Hsien Chi^{1

2}, Tzu-Ching Sung⁹

Affiliations

¹ Department of Emergency Medicine, National Cheng Kung University Hospital, National Cheng Kung University Tainan, Taiwan.
² Department of Emergency Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
³ Department of Public Health, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
⁴ National Mosquito-Borne Diseases Control Research Center, National Health Research Institutes, Tainan, Taiwan.
⁵ National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Tainan, Taiwan.
⁶ Division of Geriatrics, Department of Internal Medicine, National Cheng Kung University Hospital, Tainan, Taiwan.
⁷ Division of Geriatrics, Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
⁸ Department of Pediatrics, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
⁹ Department of Health Care Management, University of Kang Ning, Tainan, Taiwan.

Abstract

Dengue infection is a major health problem in tropical and subtropical countries. A prospective observational study in a university-affiliated hospital was conducted between August 2015 and September 2015. Patients who visited the emergency department (ED) with a presentation of any symptoms of dengue were eligible for the dengue non-structural protein 1 (NS1), IgM/IgG rapid immunochromatographic tests and real-time polymerase chain reaction (RT-PCR) to evaluate the performance of the rapid tests. Considering the RT-PCR as the gold standard for the dengue diagnosis, the ideal primary results of sensitivity (80-100%), specificity (60-84%), positive predicted value(75%-95%), and negative predicted value (70-100%) suggested that the NS1-based test with or without a combination of IgM and IgG tests have good diagnostic performances in detecting dengue infections, even in the afebrile or elderly populations.

Publication types

Observational Study

MeSH terms

Adolescent
Adult
Aged
Aged, 80 and over
Child
Child, Preschool
Chromatography, Affinity / methods*
Cities
Dengue / diagnosis*
Dengue / epidemiology
Epidemics
Female
Humans
Immunoglobulin G / analysis
Immunoglobulin M / analysis
Infant
Infant, Newborn
Male
Middle Aged
Prospective Studies
RNA, Viral / analysis
Reagent Kits, Diagnostic
Real-Time Polymerase Chain Reaction / methods
Sensitivity and Specificity
Young Adult

Substances

Immunoglobulin G
Immunoglobulin M
RNA, Viral
Reagent Kits, Diagnostic

Grants and funding

This work was supported in part by a grant MOST 102-2314-B-426-001- from the Ministry of Science and Technology (MOST), R.O.C., and by a grant NCKUH-10506016 from the Department of Emergency Medicine, National Cheng Kung University Hospital.