Abstract
Recent studies have shown that L-proline is a natural osmoprotectant and an antioxidant to protect cells from injuries such as that caused by freezing and thawing in many species including plant, ram sperm and human endothelial cells. Nevertheless, this nontoxic cryoprotectant has not yet been applied to mammalian oocyte vitrification. In this study we evaluated the efficiency and safety of the new cryoprotectant in oocyte vitrification. The results indicated that L-proline improves the survival rate of vitrified oocytes, protects mitochondrial functions and could be applied as a new cryoprotectant in mouse oocyte vitrification.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Survival / drug effects
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Cryopreservation / methods
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Cryoprotective Agents / administration & dosage
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Cryoprotective Agents / pharmacology*
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Dimethyl Sulfoxide / administration & dosage
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Embryo Culture Techniques
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Embryo Transfer
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Embryonic Development / drug effects
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Ethylene Glycol / administration & dosage
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Female
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Male
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Mice
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Mice, Inbred C57BL
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Mice, Inbred DBA
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Mice, Inbred ICR
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Mitochondria / drug effects
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Mitochondria / metabolism
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Oocytes / cytology
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Oocytes / drug effects*
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Oocytes / metabolism
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Pregnancy
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Proline / administration & dosage
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Proline / pharmacology*
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Sperm Injections, Intracytoplasmic
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Spindle Apparatus / drug effects
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Spindle Apparatus / ultrastructure
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Vitrification / drug effects*
Substances
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Cryoprotective Agents
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Proline
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Ethylene Glycol
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Dimethyl Sulfoxide