Giardia duodenalis: Number and Fluorescence Reduction Caused by the Advanced Oxidation Process (H2O2/UV)

José Roberto Guimarães; Regina Maura Bueno Franco; Regiane Aparecida Guadagnini; Luciana Urbano Dos Santos

doi:10.1155/2014/525719

Giardia duodenalis: Number and Fluorescence Reduction Caused by the Advanced Oxidation Process (H2O2/UV)

Int Sch Res Notices. 2014 Dec 4:2014:525719. doi: 10.1155/2014/525719. eCollection 2014.

Authors

José Roberto Guimarães¹, Regina Maura Bueno Franco², Regiane Aparecida Guadagnini¹, Luciana Urbano Dos Santos³

Affiliations

¹ Department of Sanitation and the Environment, School of Civil Engineering, Architecture and Urbanism, University of Campinas (UNICAMP), CP 6021, 13083-852 Campinas, SP, Brazil.
² Department of Animal Biology, Biology Institute, University of Campinas (UNICAMP), CP 6109, 13083-970 Campinas, SP, Brazil.
³ Department of Sanitation and the Environment, School of Civil Engineering, Architecture and Urbanism, University of Campinas (UNICAMP), CP 6021, 13083-852 Campinas, SP, Brazil; Padre Anchieta University Center, UNIANCHIETA, Bom Jesus de Pirapora Street 100/140, 13207-270 Jundiaí, SP, Brazil.

Abstract

This study evaluated the effect of peroxidation assisted by ultraviolet radiation (H2O2/UV), which is an advanced oxidation process (AOP), on Giardia duodenalis cysts. The cysts were inoculated in synthetic and surface water using a concentration of 12 g H2O2 L(-1) and a UV dose (λ = 254 nm) of 5,480 mJcm(-2). The aqueous solutions were concentrated using membrane filtration, and the organisms were observed using a direct immunofluorescence assay (IFA). The AOP was effective in reducing the number of G. duodenalis cysts in synthetic and surface water and was most effective in reducing the fluorescence of the cyst walls that were present in the surface water. The AOP showed a higher deleterious potential for G. duodenalis cysts than either peroxidation (H2O2) or photolysis (UV) processes alone.