Generation of human pluripotent stem cell lines with suppressed expression of the Notch ligand DLL4 using short hairpin RNAs

Stem Cell Res. 2016 May;16(3):735-9. doi: 10.1016/j.scr.2016.04.005. Epub 2016 Apr 16.

Authors

Federico González-Pozas¹, Rosa Montes¹, Lourdes López-Onieva¹, Tamara Romero¹, Joan Domingo-Reinés¹, Pedro J Real¹, Verónica Ramos-Mejía¹, Verónica Ayllón²

Affiliations

¹ Gene Regulation, Stem Cells and Development Group, Department of Genomic Oncology, GENYO - Centre for Genomics and Oncological Research - Pfizer/University of Granada/Junta de Andalucía, PTS Granada. 18016. Granada, Spain.
² Gene Regulation, Stem Cells and Development Group, Department of Genomic Oncology, GENYO - Centre for Genomics and Oncological Research - Pfizer/University of Granada/Junta de Andalucía, PTS Granada. 18016. Granada, Spain. Electronic address: veronica.ayllon@genyo.es.

PMID: 27346199
DOI: 10.1016/j.scr.2016.04.005

No abstract available

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adaptor Proteins, Signal Transducing
Biomarkers / metabolism
Calcium-Binding Proteins
Cell Culture Techniques / methods*
Cell Line
Humans
Intercellular Signaling Peptides and Proteins / metabolism*
Ligands
Pluripotent Stem Cells / cytology*
Pluripotent Stem Cells / metabolism*
RNA, Small Interfering / metabolism*
Receptors, Notch / metabolism

Substances

Adaptor Proteins, Signal Transducing
Biomarkers
Calcium-Binding Proteins
DLL4 protein, human
Intercellular Signaling Peptides and Proteins
Ligands
RNA, Small Interfering
Receptors, Notch