Data on the characterization of follicle-stimulating hormone monoclonal antibodies and localization in Japanese eel pituitary

Dae-Jung Kim; Chae-Won Park; Munkhzaya Byambaragchaa; Shin-Kwon Kim; Bae-Ik Lee; Hyung-Kyu Hwang; Jeong-In Myeong; Sun-Mee Hong; Myung-Hwa Kang; Kwan-Sik Min

doi:10.1016/j.dib.2016.05.069

Data on the characterization of follicle-stimulating hormone monoclonal antibodies and localization in Japanese eel pituitary

Data Brief. 2016 Jun 3:8:404-10. doi: 10.1016/j.dib.2016.05.069. eCollection 2016 Sep.

Authors

Affiliations

¹ Aquaculture Research Division, National Institute of Fisheries Science (NIFS), Busan 46083, Republic of Korea.
² Animal Biotechnology, Graduate School of Future Convergence Technology, Institute of Genetic Engineering, Hankyong National University, Anseong 17579, Republic of Korea.
³ Dept. of Research and Development, Institute of Gyeongbuk Marine Bioindustry, Ulgin, 36315, Republic of Korea.
⁴ Department of Food Science & Nutrition, Hoseo University, Asan 31499, Republic of Korea.

Abstract

Monoclonal antibodies were generated against recombinant follicle-stimulating hormone (rec-FSH) from Japanese eel Anguilla japonica; rec-FSH was produced in Escherichia coli and purified using Ni-NTA Sepharose column chromatography. In support of our recent publication, "Production and characterization of monoclonal antibodies against recombinant tethered follicle-stimulating hormone from Japanese eel Anguilla japonica" [1], it was important to characterize the specificity of eel follicle-stimulating hormone antibodies. Here, the production and ELISA system of these monoclonal antibodies are presented. The affinity-purified monoclonal antibodies specifically detected eel rec-FSH in ELISA and on western blots of rec-FSH produced from CHO cells. Immunohistochemical analysis revealed that FSH staining was specifically localized in the eel pituitary.

Keywords: FSH; Japanese eel; Monoclonal Antibody.