Characterization and expression analysis of Toll-interacting protein in common carp, Cyprinus carpio L., responding to bacterial and viral challenge

Shijuan Shan; Lei Wang; Fumiao Zhang; Yaoyao Zhu; Liguo An; Guiwen Yang

doi:10.1186/s40064-016-2293-3

Characterization and expression analysis of Toll-interacting protein in common carp, Cyprinus carpio L., responding to bacterial and viral challenge

Springerplus. 2016 May 17:5:639. doi: 10.1186/s40064-016-2293-3. eCollection 2016.

Authors

Shijuan Shan^#¹, Lei Wang^#¹, Fumiao Zhang¹, Yaoyao Zhu¹, Liguo An¹, Guiwen Yang¹

Affiliation

¹ Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life Science, Shandong Normal University, Jinan, 250014 People's Republic of China.

^# Contributed equally.

Abstract

Toll-interacting protein (Tollip) is a mediator involved in the TLRs signaling pathway which is critical for innate immune response. In the present study, a full-length Tollip cDNA was first cloned from common carp (CcTollip), which was 1284 bp in length, containing an open reading frame of 831 bp encoding a peptide of 276 amino acids. Multiple sequence alignment showed that the CcTollip shared the highest similarity with that of grass carp and zebrafish. Phylogenetically, the CcTollip clustered together well with their piscine family members. Quantitative real-time PCR analysis indicated that CcTollip was widely expressed in all tissues tested and showed up-regulation with challenges of Vibrio anguillarum and poly(I:C), suggesting that CcTollip was activated by V. anguillarum and poly(I:C). These data indicated that CcTollip might play an important role in immune response to bacterial and viral invasion.

Keywords: Common carp (Cyprinus carpio L.); Molecular cloning; Poly(I:C); Tollip; Vibrio anguillarum.