Rabbit brown adipose tissue uncoupling protein MRNA: use of only one of two polyadenylation signals in its processing

Biochem Biophys Res Commun. 1989 May 30;161(1):156-61. doi: 10.1016/0006-291x(89)91574-x.

Abstract

A cDNA containing the complete coding sequence of rabbit brown adipose tissue uncoupling protein was isolated and sequenced. The coding region is 80.6% identical to rat UCP cDNA and the protein is about 86% identical to the rat and hamster proteins. Despite the presence of 2 AATAAA polyadenylation consensus sequences in rabbit UCP cDNA, only one rabbit UCP mRNA was detected indicating that only the 3'-downstream signal is used in contrast to rat and mouse where both are used.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue, Brown / metabolism*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Carrier Proteins*
  • Ion Channels
  • Membrane Proteins / genetics*
  • Membrane Proteins / isolation & purification
  • Membrane Proteins / metabolism
  • Mitochondria / metabolism
  • Mitochondrial Proteins
  • Molecular Sequence Data
  • Poly A / genetics
  • Poly A / metabolism
  • Protein Processing, Post-Translational*
  • RNA, Messenger / metabolism*
  • Rabbits
  • Rats
  • Uncoupling Agents / genetics*
  • Uncoupling Agents / isolation & purification
  • Uncoupling Agents / metabolism
  • Uncoupling Protein 1

Substances

  • Carrier Proteins
  • Ion Channels
  • Membrane Proteins
  • Mitochondrial Proteins
  • RNA, Messenger
  • Uncoupling Agents
  • Uncoupling Protein 1
  • Poly A

Associated data

  • GENBANK/X14696