[Letter to the Editor] Comparison of small RNA next-generation sequencing with and without isolation of small RNA fraction

Tibor Nagy; András Kis; Szilárd Poliska; Endre Barta; Zoltán Havelda; Ferenc Marincs

doi:10.2144/000114423

[Letter to the Editor] Comparison of small RNA next-generation sequencing with and without isolation of small RNA fraction

Biotechniques. 2016 Jun 1;60(6):273-8. doi: 10.2144/000114423. eCollection 2016.

Authors

Tibor Nagy¹, András Kis¹, Szilárd Poliska², Endre Barta¹, Zoltán Havelda¹, Ferenc Marincs¹

Affiliations

¹ Agricultural Biotechnology Institute, National Agricultural Research and Innovation Centre, Gödöllö, Hungary.
² Genomic Medicine and Bioinformatic Core Facility, Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.

PMID: 27286803
DOI: 10.2144/000114423

Abstract

Address correspondence to Ferenc Marincs, Agricultural Biotechnology Institute, NARIC, Szent-Györgyi Albert u. 4., Gödöllö, Hungary. E-mail: marincs.ferenc@abc.naik.hu.

Keywords: Illumina sequencing; library preparation; miRNA; wheat.

Publication types

Comparative Study
Letter
Research Support, Non-U.S. Gov't

MeSH terms

High-Throughput Nucleotide Sequencing / methods*
RNA, Plant / chemistry
RNA, Plant / genetics*
RNA, Plant / isolation & purification
RNA, Small Interfering / chemistry
RNA, Small Interfering / genetics*
RNA, Small Interfering / isolation & purification*
Reproducibility of Results
Sample Size
Sensitivity and Specificity
Sequence Analysis, RNA / standards*
Triticum / genetics*

Substances

RNA, Plant
RNA, Small Interfering