Pseudo-Ligandless Click Chemistry for Oligonucleotide Conjugation

Stephanie Mack; Munira F Fouz; Sourav K Dey; Subha R Das

doi:10.1002/cpch.1

Pseudo-Ligandless Click Chemistry for Oligonucleotide Conjugation

Curr Protoc Chem Biol. 2016 Jun 2;8(2):83-95. doi: 10.1002/cpch.1.

Authors

Stephanie Mack^{1

2}, Munira F Fouz^{1

2}, Sourav K Dey^{1

2}, Subha R Das¹

Affiliations

¹ Department of Chemistry and Center for Nucleic Acids Science & Technology, Carnegie Mellon University, Pittsburgh, Pennsylvania.
² These authors contributed equally to this work.

PMID: 27258688
PMCID: PMC4940987
DOI: 10.1002/cpch.1

Abstract

Particularly for its use in bioconjugations, the copper-catalyzed (or copper-promoted) azide-alkyne cycloaddition (CuAAC) reaction or 'click chemistry', has become an essential component of the modern chemical biologist's toolbox. Click chemistry has been applied to DNA, and more recently, RNA conjugations, and the protocols presented here can be used for either. The reaction can be carried out in aqueous buffer, and uses acetonitrile as a minor co-solvent that serves as a ligand to stabilize the copper. The method also includes details on the analysis of the reaction product. © 2016 by John Wiley & Sons, Inc.

Keywords: DNA; RNA; alkyne; azide; bioconjugation; labeling; nucleic acids.

Publication types

Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural

MeSH terms

Alkynes / chemistry*
Azides / chemistry*
Click Chemistry / methods*
DNA / chemistry
Oligonucleotides / chemistry*
RNA / chemistry

Substances

Alkynes
Azides
Oligonucleotides
RNA
DNA

Grants and funding

R01 GM110414/GM/NIGMS NIH HHS/United States