In vitro-synthesized mRNA containing nucleoside modifications has great therapeutical potential to transiently express proteins with physiological importance. One such protein is photolyase which rapidly removes UV-induced DNA damages, but this enzyme is absent in humans. Here, we apply a novel mRNA-based platform to achieve functional nonhuman photolyase production in cultured human keratinocytes. Transfection of nucleoside-modified mRNA encoding photolyase leads to accelerated repair of DNA photolesions in human keratinocytes.
Keywords: Cyclobutane pyrimidine dimer-specific photolyase; DNA repair; Human keratinocytes; Nonviral gene therapy; Pseudouridine-modified mRNA; Transient transfection; Ultraviolet B.