Abstract
Two reactive sequences of the pre-S regions of hepatitis B surface antigen were synthesized chemically and used in micro-ELISAs for the assay of pre-S1 and pre-S2 antigens in serum from patients with acute and chronic hepatitis B. Pre-S1 antigen correlated well with the presence of HBV-DNA and was no longer detectable on cessation of viral replication, after natural recovery and after successful treatment with alpha-interferon. Pre-S2 proteins were also lost after treatment with alpha-interferon. The results show that the assay of pre-S1 and pre-S2 proteins in serum provides additional useful markers for assessing patients with acute and chronic hepatitis B infection and for monitoring the response to treatment with interferon.
MeSH terms
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Amino Acid Sequence
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Antibodies, Monoclonal / immunology
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DNA, Viral / isolation & purification
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Enzyme-Linked Immunosorbent Assay
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Hepatitis B / diagnosis*
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Hepatitis B / therapy
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Hepatitis B Antibodies / biosynthesis
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Hepatitis B Surface Antigens / analysis
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Hepatitis B Surface Antigens / chemical synthesis*
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Hepatitis B Surface Antigens / immunology
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Hepatitis B virus / immunology
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Hepatitis B virus / isolation & purification
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Humans
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Interferon Type I / pharmacology
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Interferon Type I / therapeutic use
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Molecular Sequence Data
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Nucleic Acid Hybridization
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Protein Precursors / analysis
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Protein Precursors / chemical synthesis*
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Protein Precursors / immunology
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Viral Envelope Proteins / analysis
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Viral Envelope Proteins / chemical synthesis*
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Viral Envelope Proteins / immunology
Substances
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Antibodies, Monoclonal
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DNA, Viral
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Hepatitis B Antibodies
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Hepatitis B Surface Antigens
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Interferon Type I
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Protein Precursors
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Viral Envelope Proteins
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presurface protein 1, hepatitis B surface antigen
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presurface protein 2, hepatitis B surface antigen