Direct Modulation of Heterotrimeric G Protein-coupled Signaling by a Receptor Kinase Complex

J Biol Chem. 2016 Jul 1;291(27):13918-13925. doi: 10.1074/jbc.C116.736702. Epub 2016 May 27.

Abstract

Plants and some protists have heterotrimeric G protein complexes that activate spontaneously without canonical G protein-coupled receptors (GPCRs). In Arabidopsis, the sole 7-transmembrane regulator of G protein signaling 1 (AtRGS1) modulates the G protein complex by keeping it in the resting state (GDP-bound). However, it remains unknown how a myriad of biological responses is achieved with a single G protein modulator. We propose that in complete contrast to G protein activation in animals, plant leucine-rich repeat receptor-like kinases (LRR RLKs), not GPCRs, provide this discrimination through phosphorylation of AtRGS1 in a ligand-dependent manner. G protein signaling is directly activated by the pathogen-associated molecular pattern flagellin peptide 22 through its LRR RLK, FLS2, and co-receptor BAK1.

Keywords: Arabidopsis thaliana; AtRGS1; BAK1; FLS2; G protein; flg22; phosphorylation; receptor protein serine/threonine kinase; receptor-like kinases; regulator of G protein signaling (RGS).

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / genetics
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Flagellin / pharmacology
  • Peptides / pharmacology
  • Phosphorylation / drug effects
  • Phosphorylation / physiology
  • Protein Serine-Threonine Kinases / genetics
  • Protein Serine-Threonine Kinases / metabolism*
  • RGS Proteins / genetics
  • RGS Proteins / metabolism*
  • Signal Transduction / drug effects
  • Signal Transduction / physiology*

Substances

  • Arabidopsis Proteins
  • Peptides
  • RGS Proteins
  • RGS1 protein, Arabidopsis
  • Flagellin
  • BAK1 protein, Arabidopsis
  • Protein Serine-Threonine Kinases