Nipah Virus C Protein Recruits Tsg101 to Promote the Efficient Release of Virus in an ESCRT-Dependent Pathway

PLoS Pathog. 2016 May 20;12(5):e1005659. doi: 10.1371/journal.ppat.1005659. eCollection 2016 May.

Abstract

The budding of Nipah virus, a deadly member of the Henipavirus genus within the Paramyxoviridae, has been thought to be independent of the host ESCRT pathway, which is critical for the budding of many enveloped viruses. This conclusion was based on the budding properties of the virus matrix protein in the absence of other virus components. Here, we find that the virus C protein, which was previously investigated for its role in antagonism of innate immunity, recruits the ESCRT pathway to promote efficient virus release. Inhibition of ESCRT or depletion of the ESCRT factor Tsg101 abrogates the C enhancement of matrix budding and impairs live Nipah virus release. Further, despite the low sequence homology of the C proteins of known henipaviruses, they all enhance the budding of their cognate matrix proteins, suggesting a conserved and previously unknown function for the henipavirus C proteins.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • Blotting, Western
  • DNA-Binding Proteins / metabolism*
  • Endosomal Sorting Complexes Required for Transport / metabolism*
  • HEK293 Cells
  • Henipavirus Infections / metabolism*
  • Humans
  • Immunoprecipitation
  • Microscopy, Confocal
  • Microscopy, Electron, Transmission
  • Nipah Virus / physiology*
  • Phosphoproteins / metabolism*
  • Transcription Factors / metabolism*
  • Viral Proteins / metabolism*
  • Virus Release / physiology*

Substances

  • C protein, Nipah virus
  • DNA-Binding Proteins
  • Endosomal Sorting Complexes Required for Transport
  • Phosphoproteins
  • Transcription Factors
  • Tsg101 protein
  • Viral Proteins