Assessment of serum thiol/disulfide homeostasis in multiple myeloma patients by a new method

Redox Rep. 2017 Nov;22(6):246-251. doi: 10.1080/13510002.2016.1180100. Epub 2016 May 19.

Abstract

Objectives: The etiology of multiple myeloma (MM) is not exactly known. This study investigated the role of thiol/disulfide homeostasis in the etiopathogenesis of MM.

Methods: Some 50 patients with MM (aged 39-84 years) and 50 sex-matched healthy volunteer controls (aged 50-91 years) participated in this study. Venous blood samples were collected, and levels of native thiols, total thiols, and disulfide were measured.

Results: Native and total thiol levels in the control group were determined to be higher than in the study and patient groups (P<0.001). Disulfide levels were found to be higher in the control group than in the study group and higher in newly diagnosed patients than in outpatients who were undergoing treatment (P=0.002). The ratios of thiol levels were found to be similar in both the study and control groups (P>0.05).

Discussion: The results of the study show that although there was a decrease in the levels of disulfide, native thiol, and total thiol, the balance of thiol/disulfide was maintained. This is the first study to research the homeostasis of dynamic thiol/disulfide from the perspective of the new method that was used. We hope that this study will encourage and facilitate further studies in this area.

Keywords: Disulfide; Multiple myeloma; Oxidative stress; Thiol.

MeSH terms

  • Aged
  • Aged, 80 and over
  • Case-Control Studies
  • Disulfides / metabolism*
  • Female
  • Homeostasis / genetics
  • Homeostasis / physiology
  • Humans
  • Male
  • Middle Aged
  • Multiple Myeloma / metabolism*
  • Oxidative Stress / physiology
  • Sulfhydryl Compounds / metabolism*

Substances

  • Disulfides
  • Sulfhydryl Compounds