A novel transcription unit within the exon sequence of the rat insulin like growth factor II gene

T Matsuguchi; K Takahashi; T Ueno; H Endo; M Yamamoto

A novel transcription unit within the exon sequence of the rat insulin like growth factor II gene

Biochem Int. 1989 Jan;18(1):71-9.

Authors

T Matsuguchi¹, K Takahashi, T Ueno, H Endo, M Yamamoto

Affiliation

¹ Department of Biochemistry, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.

PMID: 2719715

Abstract

We identified a transcription unit within a single exon of the rat insulin like growth factor II (rIGFII) gene by a combination of Northern blotting, S1 nuclease mapping and primer extension analyses. Among multiple mRNA products of rIGFII, the 1.8kb transcript has a discrete 5' terminus and is encoded exclusively in a single 3'-extreme exon with no trace of the coding region. The upstream region of the 5' terminus contains a completely matching GC-rich 16bp palindrome and several sequence motifs highly homologous to the consensus sequences of binding sites for transcriptional regulatory proteins AP2, H2TF1 and NF-kappa B.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Blotting, Northern
Cell Line
Exons*
Genes*
Insulin-Like Growth Factor II / genetics*
Liver Neoplasms, Experimental / metabolism
Molecular Sequence Data
Nucleic Acid Conformation
RNA, Messenger / genetics
Rats
Somatomedins / genetics*
Transcription, Genetic*

Substances

RNA, Messenger
Somatomedins
Insulin-Like Growth Factor II