Unprecedented staining of polar lipids by a luminescent rhenium complex revealed by FTIR microspectroscopy in adipocytes

C A Bader; E A Carter; A Safitri; P V Simpson; P Wright; S Stagni; M Massi; P A Lay; D A Brooks; S E Plush

doi:10.1039/c6mb00242k

Unprecedented staining of polar lipids by a luminescent rhenium complex revealed by FTIR microspectroscopy in adipocytes

Mol Biosyst. 2016 Jun 21;12(7):2064-8. doi: 10.1039/c6mb00242k.

Authors

C A Bader¹, E A Carter², A Safitri², P V Simpson³, P Wright³, S Stagni⁴, M Massi³, P A Lay², D A Brooks¹, S E Plush¹

Affiliations

¹ Mechanisms in Cell Biology and Disease Research Group, School of Pharmacy and Medical Sciences/Sansom Institute for Health Research, University of South Australia, Adelaide, Australia. sally.plush@unisa.edu.au.
² Vibrational Spectroscopy Core Facility and School of Chemistry, The University of Sydney, Sydney, Australia.
³ School of Chemistry, Curtin University, Perth, Australia.
⁴ Department of Industrial Chemistry "Toso Montanari", University of Bologna, Bologna, Italy.

PMID: 27170554
DOI: 10.1039/c6mb00242k

Abstract

Fourier transform infrared (FTIR) microspectroscopy and confocal imaging have been used to demonstrate that the neutral rhenium(i) tricarbonyl 1,10-phenanthroline complex bound to 4-cyanophenyltetrazolate as the ancillary ligand is able to localise in regions with high concentrations of polar lipids such as phosphatidylethanolamine (PE), sphingomyelin, sphingosphine and lysophosphatidic acid (LPA) in mammalian adipocytes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3T3-L1 Cells
Adipocytes / metabolism*
Animals
Lipid Metabolism*
Lipids* / chemistry
Luminescent Agents*
Mice
Rhenium*
Spectroscopy, Fourier Transform Infrared*

Substances

Lipids
Luminescent Agents
Rhenium