Contribution of low- and high-flux capillaries to slow hemodynamic fluctuations in the cerebral cortex of mice

J Cereb Blood Flow Metab. 2016 Aug;36(8):1351-6. doi: 10.1177/0271678X16649195. Epub 2016 May 10.

Abstract

We employed optical coherence tomography to measure cerebral cortical capillary red blood cell (RBC) flux in mice. The results suggest that baseline-flux weakly depends on cortical depth. Furthermore, under hypercapnia, low baseline-flux capillaries exhibit greater flux increases while the higher ones saturate, resulting in RBC-flux homogenization. Power-spectrum analysis indicates that higher flux capillaries saw greater flux variability in the low-frequency range (0.01-0.1 Hz) both at baseline and during hypercapnia. These results suggest that lower baseline-flux capillaries have more reserve to deliver oxygen with increased blood flow; but higher ones more strongly impact the low-frequency fluctuations associated with BOLD fMRI measurements of resting state functional connectivity.

Keywords: Capillaries; OCT; flux; low frequency; oscillation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Flow Velocity / physiology*
  • Capillaries / diagnostic imaging
  • Capillaries / physiopathology*
  • Cerebral Angiography
  • Cerebral Cortex / blood supply*
  • Cerebral Cortex / diagnostic imaging
  • Cerebrovascular Circulation / physiology*
  • Erythrocytes / cytology
  • Erythrocytes / physiology
  • Hypercapnia / physiopathology
  • Hypoxia / physiopathology
  • Male
  • Mice, Inbred Strains
  • Microcirculation / physiology
  • Tomography, Optical Coherence