Furan-PNA: a mildly inducible irreversible interstrand crosslinking system targeting single and double stranded DNA

Chem Commun (Camb). 2016 May 25;52(42):6930-3. doi: 10.1039/c6cc02062c. Epub 2016 May 5.

Abstract

We here report on the design and synthesis of tailor-made furan-modified peptide nucleic acid (PNA) probes for covalent targeting of single stranded DNA through a crosslinking strategy. After introducing furan-containing building blocks into a PNA sequence, hybridization and furan-oxidation based crosslinking to DNA is investigated. The structure of the crosslinked products is characterized and preliminary investigations concerning the application of these systems to double stranded DNA are shown.

MeSH terms

  • Base Sequence
  • DNA, Single-Stranded / chemistry*
  • DNA, Single-Stranded / genetics
  • Furans / chemistry*
  • Peptide Nucleic Acids / chemistry*

Substances

  • DNA, Single-Stranded
  • Furans
  • Peptide Nucleic Acids