Transcription-induced DNA supercoiling: New roles of intranucleosomal DNA loops in DNA repair and transcription

N S Gerasimova; N A Pestov; O I Kulaeva; D J Clark; V M Studitsky

doi:10.1080/21541264.2016.1182240

Transcription-induced DNA supercoiling: New roles of intranucleosomal DNA loops in DNA repair and transcription

Transcription. 2016 May 26;7(3):91-5. doi: 10.1080/21541264.2016.1182240. Epub 2016 Apr 26.

Authors

N S Gerasimova¹, N A Pestov², O I Kulaeva^{1

3}, D J Clark⁴, V M Studitsky^{1

2

3}

Affiliations

¹ a Biology Faculty, Lomonosov Moscow State University , Moscow , Russia.
² b Department of Pharmacology , Rutgers-Robert Wood Johnson Medical School , Piscataway , NJ , USA.
³ c Cancer Epigenetics Program, Fox Chase Cancer Center , Philadelphia , PA , USA.
⁴ d Division of Developmental Biology, National Institute of Child Health and Human Development, National Institutes of Health , Bethesda , MD , USA.

Abstract

RNA polymerase II (Pol II) transcription through chromatin is accompanied by formation of small intranucleosomal DNA loops. Pol II captured within a small loop drives accumulation of DNA supercoiling, facilitating further transcription. DNA breaks relieve supercoiling and induce Pol II arrest, allowing detection of DNA damage hidden in chromatin structure.

Keywords: DNA repair; DNA supercoiling; RNA polymerase II; chromatin; elongation; intermediates; intranucleosomal DNA loops; mechanism; nucleosome; single strand DNA breaks; structure; transcription.

Publication types

Research Support, N.I.H., Extramural
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chromatin / chemistry
DNA / chemistry*
DNA / genetics
DNA Damage
DNA Repair*
Humans
Nucleic Acid Conformation
RNA Polymerase II / metabolism
Transcription, Genetic*

Substances

Chromatin
DNA
RNA Polymerase II

Abstract

Publication types

MeSH terms

Substances

Grants and funding