BDNF promotes the axonal regrowth after sciatic nerve crush through intrinsic neuronal capability upregulation and distal portion protection

Neurosci Lett. 2016 May 16:621:1-8. doi: 10.1016/j.neulet.2016.04.006. Epub 2016 Apr 4.

Abstract

Nowadays peripheral nerve injurie occurs more common, the outcome is often poor because of the ineffective treatment. Recent researches revealed the duration of BDNF administration acts a positive role during the nerve regeneration, but its potential mechanisms beneath the behavioral recovery and axonal regrowth after peripheral nerve injury are still controversial. To observe the potential mechanisms we established sciatic nerve injury model and detected the expression of several axonal regeneration and function related genes. The results showed that, BDNF promotes axonal regrowth through increasing the activation of neuronal intrinsic growth capacity and strengthening the deference effects against distal portion atrophy. To further study, we determined the expression of protein associated to neuronal intrinsic growth capacity and investigated the ultrastructure of the distal portion of the injured nerve were analyzed. These data revealed that BDNF triggers multiple effects including neuronal intrinsic growth capacity improvement and distal portion atrophy protection to promote behavioral recovery following sciatic nerve crush injury in mouse.

Keywords: BDNF; Gap43; SCI; TEM; Toe spreading; cAMP.

MeSH terms

  • Animals
  • Axons / physiology*
  • Brain-Derived Neurotrophic Factor / pharmacology
  • Brain-Derived Neurotrophic Factor / physiology*
  • Male
  • Mice, Inbred C57BL
  • Nerve Regeneration*
  • Neurons / physiology*
  • Peripheral Nerve Injuries / metabolism
  • Peripheral Nerve Injuries / pathology
  • Peripheral Nerve Injuries / physiopathology*
  • Sciatic Nerve / injuries*
  • Sciatic Nerve / pathology
  • Sciatic Nerve / physiopathology

Substances

  • Brain-Derived Neurotrophic Factor