Evaluation of Multiple Methods for Detection of Gastrointestinal Colonization of Carbapenem-Resistant Organisms from Rectal Swabs

J Clin Microbiol. 2016 Jun;54(6):1664-1667. doi: 10.1128/JCM.00548-16. Epub 2016 Apr 6.

Abstract

Rectal swabs from high-risk patients were screened for carbapenem-resistant organisms (CROs) using several methods. The direct MacConkey plate method was the most sensitive for CROs (95%), while chromID CARBA and the Check-Direct CPE screen assay were the most sensitive for the detection of carbapenemase-producing organisms (CPOs) (100%; all blaKPC). All methods had a specificity of >90% for CROs, and for CPOs, the specificity ranged from 85 to 98%. Broth enrichment methods performed poorly compared to direct inoculation methods, negating the need for the broth enrichment step.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Bacterial Proteins / analysis
  • Bacteriological Techniques / methods*
  • Carbapenems / pharmacology*
  • Gram-Negative Bacteria / drug effects*
  • Gram-Negative Bacteria / isolation & purification*
  • Gram-Negative Bacterial Infections / diagnosis*
  • Gram-Negative Bacterial Infections / microbiology
  • Humans
  • Rectum / microbiology*
  • Sensitivity and Specificity
  • beta-Lactam Resistance*
  • beta-Lactamases / analysis

Substances

  • Bacterial Proteins
  • Carbapenems
  • beta-Lactamases
  • carbapenemase

Grants and funding

This study was funded in part by the Centers for Diseases Control and Prevention's Prevention Epicenters Program through collaborative agreement number 1 U54 CK000447-001 and by the Fisher Center Discovery Program award supported by the Sherrilyn and Ken Fisher Center for Environmental Infectious Diseases, Division of Infectious Diseases of the Johns Hopkins University School of Medicine. The contents of this work are solely the responsibility of the authors and do not necessarily represent the official views of the Centers for Disease Control and Prevention, the Department of Health and Human Services, or the Sherrilyn and Ken Fisher Center for Environmental Infectious Diseases. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.