Combination of Bioactive Polymeric Membranes and Stem Cells for Periodontal Regeneration: In Vitro and In Vivo Analyses

Flávia Gonçalves; Míriam Santos de Moraes; Lorraine Braga Ferreira; Ana Cláudia Oliveira Carreira; Patrícia Mayumi Kossugue; Letícia Cristina Cidreira Boaro; Ricardo Bentini; Célia Regina da Silva Garcia; Mari Cleide Sogayar; Victor Elias Arana-Chavez; Luiz Henrique Catalani

doi:10.1371/journal.pone.0152412

Combination of Bioactive Polymeric Membranes and Stem Cells for Periodontal Regeneration: In Vitro and In Vivo Analyses

PLoS One. 2016 Mar 31;11(3):e0152412. doi: 10.1371/journal.pone.0152412. eCollection 2016.

Affiliations

¹ Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, São Paulo, SP, Brasil, 05508-000.
² Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brasil, 05508-090.
³ Departamento de Biomateriais e Biologia Oral, Faculdade de Odontologia, Universidade de São Paulo, São Paulo, SP, Brasil, 05508-000.
⁴ NUCEL/NETCEM-Núcleo de Terapia Celular e Molecular, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brasil, 05360-130.
⁵ Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP, Brasil, 05508-000.

Abstract

Regeneration of periodontal tissues requires a concerted effort to obtain consistent and predictable results in vivo. The aim of the present study was to test a new family of bioactive polymeric membranes in combination with stem cell therapy for periodontal regeneration. In particular, the novel polyester poly(isosorbide succinate-co-L-lactide) (PisPLLA) was compared with poly(L-lactide) (PLLA). Both polymers were combined with collagen (COL), hydroxyapatite (HA) and the growth factor bone morphogenetic protein-7 (BMP7), and their osteoinductive capacity was evaluated via in vitro and in vivo experiments. Membranes composed of PLLA/COL/HA or PisPLLA/COL/HA were able to promote periodontal regeneration and new bone formation in fenestration defects in rat jaws. According to quantitative real-time polymerase chain reaction (qRT-PCR) and Alizarin Red assays, better osteoconductive capacity and increased extracellular mineralization were observed for PLLA/COL/HA, whereas better osteoinductive properties were associated with PisPLLA/COL/HA. We concluded that membranes composed of either PisPLLA/COL/HA or PLLA/COL/HA present promising results in vitro as well as in vivo and that these materials could be potentially applied in periodontal regeneration.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bone Morphogenetic Protein 7 / chemistry
Bone Substitutes / chemistry
Bone Substitutes / pharmacology
Cell Differentiation / drug effects
Cell Proliferation / drug effects
Cells, Cultured
Collagen / chemistry
Core Binding Factor Alpha 1 Subunit / genetics
Core Binding Factor Alpha 1 Subunit / metabolism
Dental Pulp / cytology
Durapatite / chemistry
Humans
Osteogenesis / drug effects
Osteopontin / genetics
Osteopontin / metabolism
Periodontium / pathology
Periodontium / physiology*
Polyesters / chemistry
Polymers / chemistry*
Rats
Rats, Wistar
Regeneration*
Stem Cells / cytology*
Stem Cells / metabolism
Tissue Engineering
Tissue Scaffolds

Substances

Bone Morphogenetic Protein 7
Bone Substitutes
Core Binding Factor Alpha 1 Subunit
Polyesters
Polymers
Osteopontin
poly(lactide)
Collagen
Durapatite

Grants and funding

The authors FG, RB and LHC would like to thank CNPq and FAPESP (2010/17698-2 and 2011/21442-6) for funding. Additionally, MM and CRSG acknowledge funding from CNPq and FAPESP (2011/51295-5), and ACOC, PMK and MCS acknowledge funding from BNDES, CNPq, FAPESP, FINEP, MCTI and MS-DECIT. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.