In vivo risk evaluation of carbon-coated iron carbide nanoparticles based on short- and long-term exposure scenarios

Nanomedicine (Lond). 2016 Apr;11(7):783-96. doi: 10.2217/nnm.16.22. Epub 2016 Mar 16.

Abstract

Background: While carbon-encapsulated iron carbide nanoparticles exhibit strong magnetic properties appealing for biomedical applications, potential side effects of such materials remain comparatively poorly understood. Here, we assess the effects of iron-based nanoparticles in an in vivo long-term study in mice with observation windows between 1 week and 1 year.

Materials & methods: Functionalized (PEG or IgG) carbon-encapsulated platinum-spiked iron carbide nanoparticles were injected intravenously in mice (single or repeated dose administration).

Results: One week after administration, magnetic nanoparticles were predominantly localized in organs of the reticuloendothelial system, particularly the lung and liver. After 1 year, particles were still present in these organs, however, without any evident tissue alterations, such as inflammation, fibrosis, necrosis or carcinogenesis. Importantly, reticuloendothelial system organs presented with normal function.

Conclusion: This long-term exposure study shows high in vivo compatibility of intravenously applied carbon-encapsulated iron nanoparticles suggesting continuing investigations on such materials for biomedical applications.

Keywords: blood purification; long-term study; magnetic nanoparticles; targeted drug delivery.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carbon / administration & dosage
  • Carbon / adverse effects*
  • Carbon / chemistry
  • Carbon / pharmacokinetics
  • Carbon Compounds, Inorganic / administration & dosage
  • Carbon Compounds, Inorganic / adverse effects*
  • Carbon Compounds, Inorganic / chemistry
  • Carbon Compounds, Inorganic / pharmacokinetics
  • Coated Materials, Biocompatible / administration & dosage
  • Coated Materials, Biocompatible / adverse effects*
  • Coated Materials, Biocompatible / chemistry
  • Drug Carriers / administration & dosage
  • Drug Carriers / adverse effects*
  • Drug Carriers / chemistry
  • Drug Carriers / pharmacokinetics
  • Female
  • Iron Compounds / administration & dosage
  • Iron Compounds / adverse effects*
  • Iron Compounds / chemistry
  • Iron Compounds / pharmacokinetics
  • Liver / drug effects
  • Liver / metabolism
  • Liver / ultrastructure
  • Lung / drug effects
  • Lung / metabolism
  • Lung / ultrastructure
  • Magnets / adverse effects
  • Magnets / chemistry
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Nanoparticles / administration & dosage
  • Nanoparticles / adverse effects*
  • Nanoparticles / analysis
  • Nanoparticles / chemistry

Substances

  • Carbon Compounds, Inorganic
  • Coated Materials, Biocompatible
  • Drug Carriers
  • Iron Compounds
  • iron carbide
  • Carbon