Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Accelerates Pathogen Identification and May Confer Benefit in the Outcome of Peritoneal Dialysis-Related Peritonitis

Wei-Hung Lin; Jyh-Chang Hwang; Chin-Chung Tseng; Yu-Tzu Chang; An-Bang Wu; Jing-Jou Yan; Jiunn-Jong Wu; Ming-Cheng Wang

doi:10.1128/JCM.03378-15

Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Accelerates Pathogen Identification and May Confer Benefit in the Outcome of Peritoneal Dialysis-Related Peritonitis

J Clin Microbiol. 2016 May;54(5):1381-3. doi: 10.1128/JCM.03378-15. Epub 2016 Feb 24.

Authors

Wei-Hung Lin¹, Jyh-Chang Hwang², Chin-Chung Tseng³, Yu-Tzu Chang³, An-Bang Wu³, Jing-Jou Yan⁴, Jiunn-Jong Wu⁵, Ming-Cheng Wang⁶

Affiliations

¹ Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
² Division of Nephrology, Chi Mei Medical Center, Tainan, Taiwan.
³ Division of Nephrology, Department of Internal Medicine, National Cheng Kung University Hospital, Tainan, Taiwan.
⁴ Department of Pathology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
⁵ Department of Medical Laboratory Science and Biotechnology, College of Medicine, National Cheng Kung University, Tainan, Taiwan Department of Biotechnology and Laboratory Science in Medicine, School of Biomedical Science and Engineering, National Yang-Ming University, Taipei, Taiwan jjwu@mail.ncku.edu.tw wangmc@mail.ncku.edu.tw.
⁶ Division of Nephrology, Department of Internal Medicine, National Cheng Kung University Hospital, Tainan, Taiwan Institute of Clinical Pharmacy and Pharmaceutical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan jjwu@mail.ncku.edu.tw wangmc@mail.ncku.edu.tw.

Abstract

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and conventional standard methods were compared for time to pathogen identification and impact on clinical outcomes in peritoneal dialysis-related peritonitis patients. The MALDI-TOF MS method identified the causative microorganisms earlier (average time saved, 64 h for all pathogens), and patients had a shorter hospital stay (mean ± standard deviation, 5.2 ± 4.8 days versus 8.2 ± 4.5 days, P = 0.001).

Publication types

Comparative Study
Evaluation Study

MeSH terms

Female
Humans
Male
Microbiological Techniques / methods*
Peritoneal Dialysis / adverse effects*
Peritonitis / diagnosis*
Retrospective Studies
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Time Factors