Intracellular Delivery by Shape Anisotropic Magnetic Particle-Induced Cell Membrane Cuts

Ming-Yu Lin; Yi-Chien Wu; Ji-Ann Lee; Kuan-Wen Tung; Jessica Zhou; Michael A Teitell; J Andrew Yeh; Pei Yu Chiou

doi:10.1177/2211068216630743

Intracellular Delivery by Shape Anisotropic Magnetic Particle-Induced Cell Membrane Cuts

J Lab Autom. 2016 Aug;21(4):548-56. doi: 10.1177/2211068216630743. Epub 2016 Feb 16.

Authors

Ming-Yu Lin¹, Yi-Chien Wu², Ji-Ann Lee³, Kuan-Wen Tung², Jessica Zhou², Michael A Teitell⁴, J Andrew Yeh¹, Pei Yu Chiou⁵

Affiliations

¹ Instrument Technology Research Center, National Applied Research Laboratories, Hsinchu, Taiwan.
² Mechanical and Aerospace Engineering Department, University of California at Los Angeles, Los Angeles, CA, USA.
³ Department of Biological Chemistry, University of California at Los Angeles, Los Angeles, CA, USA.
⁴ Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA, USA California NanoSystems Institute (CNSI), University of California at Los Angeles, Los Angeles, CA, USA.
⁵ Mechanical and Aerospace Engineering Department, University of California at Los Angeles, Los Angeles, CA, USA Department of Bioengineering, University of California at Los Angeles, Los Angeles, CA, USA California NanoSystems Institute (CNSI), University of California at Los Angeles, Los Angeles, CA, USA pychiou@seas.ucla.edu.

PMID: 26882924
DOI: 10.1177/2211068216630743

Abstract

Introducing functional macromolecules into a variety of living cells is challenging but important for biology research and cell-based therapies. We report a novel cell delivery platform based on rotating shape anisotropic magnetic particles (SAMPs), which make very small cuts on cell membranes for macromolecule delivery with high efficiency and high survivability. SAMP delivery is performed by placing commercially available nickel powder onto cells grown in standard cell culture dishes. Application of a uniform magnetic field causes the magnetic particles to rotate because of mechanical torques induced by shape anisotropic magnetization. Cells touching these rotating particles are nicked, which generates transient membrane pores that enable the delivery of macromolecules into the cytosol of cells. Calcein dye, 3 and 40 kDa dextran polymers, a green fluorescence protein (GFP) plasmid, siRNA, and an enzyme (β-lactamase) were successfully delivered into HeLa cells, primary normal human dermal fibroblasts (NHDFs), and mouse cortical neurons that can be difficult to transfect. The SAMP approach offers several advantages, including easy implementation, low cost, high throughput, and efficient delivery of a broad range of macromolecules. Collectively, SAMP delivery has great potential for a broad range of academic and industrial applications.

Keywords: enzyme; gene therapy; intracellular delivery; mouse cortical neurons; siRNA.

MeSH terms

Animals
Cell Membrane / metabolism*
Cells, Cultured
Drug Delivery Systems / methods*
Genetic Therapy / methods
Humans
Macromolecular Substances / metabolism*
Magnetics / methods*
Mice
Transfection / methods

Substances

Macromolecular Substances

Abstract

MeSH terms

Substances

Grants and funding