Serial measurements of fetal bladder volume were obtained by real-time ultrasonography at 2- to 5-minute intervals, and the hourly fetal urine production rate was calculated. The mean hourly fetal urine production rate increased from 5 ml/hr at 20 weeks' gestation to 51 ml/hr at 40 weeks. These values are double those reported in previous studies that measured fetal bladder volumes at 15- to 30-minute intervals because the cycle length is shorter than previously thought.