Characterization of long non-coding RNA-associated ceRNA network to reveal potential prognostic lncRNA biomarkers in human ovarian cancer

Oncotarget. 2016 Mar 15;7(11):12598-611. doi: 10.18632/oncotarget.7181.

Abstract

Accumulating evidence has underscored the important roles of long non-coding RNAs (lncRNAs) acting as competing endogenous RNAs (ceRNAs) in cancer initiation and progression. In this study, we used an integrative computational method to identify miRNA-mediated ceRNA crosstalk between lncRNAs and mRNAs, and constructed global and progression-related lncRNA-associated ceRNA networks (LCeNETs) in ovarian cancer (OvCa) based on "ceRNA hypothesis". The constructed LCeNETs exhibited small world, modular architecture and high functional specificity for OvCa. Known OvCa-related genes tended to be hubs and occurred preferentially in the functional modules. Ten lncRNA ceRNAs were identified as potential candidates associated with stage progression in OvCa using ceRNA-network driven method. Finally, we developed a ten-lncRNA signature which classified patients into high- and low-risk subgroups with significantly different survival outcomes. Our study will provide novel insight for better understanding of ceRNA-mediated gene regulation in progression of OvCa and facilitate the identification of novel diagnostic and therapeutic lncRNA ceRNAs for OvCa.

Keywords: biomarker; competing endogenous RNA; long non-coding RNA; ovarian cancer.

MeSH terms

  • Biomarkers, Tumor / genetics*
  • Computational Biology / methods
  • Female
  • Gene Expression Regulation, Neoplastic / genetics*
  • Gene Regulatory Networks
  • Humans
  • MicroRNAs / genetics*
  • Ovarian Neoplasms / genetics*
  • Prognosis
  • RNA, Long Noncoding / genetics*
  • Receptor Cross-Talk / physiology

Substances

  • Biomarkers, Tumor
  • MicroRNAs
  • RNA, Long Noncoding