C-C chemokine receptor type 4 antagonist Compound 22 ameliorates experimental autoimmune encephalomyelitis

J Neuroimmunol. 2016 Feb 15:291:54-8. doi: 10.1016/j.jneuroim.2015.12.011. Epub 2015 Dec 24.

Abstract

Chemokines and chemokine receptors play important roles in the immune response. We previously reported the pathogenic role of C-C chemokine receptor type 4 (CCR4) in experimental autoimmune encephalomyelitis (EAE). Here, we examined whether CCR4 antagonism modulates the disease course of EAE. Wild-type and CCR4-knockout mice were induced EAE and were administered Compound 22, an antagonist of CCR4. Compound 22 significantly ameliorated the severity of EAE in wild-type mice, but not in the CCR4-knockout mice. Compound 22 inhibited Th1 and Th17 polarization of antigen-induced T-cell responses. Therefore, CCR4 antagonists might be potential therapeutic agents for multiple sclerosis.

Keywords: Chemokine receptor; Experimental autoimmune encephalomyelitis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Proliferation / drug effects
  • Cell Proliferation / genetics
  • Cytokines / metabolism
  • Dose-Response Relationship, Drug
  • Encephalomyelitis, Autoimmune, Experimental / chemically induced
  • Encephalomyelitis, Autoimmune, Experimental / drug therapy*
  • Encephalomyelitis, Autoimmune, Experimental / genetics
  • Female
  • Fluoresceins / therapeutic use*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Myelin-Oligodendrocyte Glycoprotein / immunology
  • Myelin-Oligodendrocyte Glycoprotein / toxicity
  • Peptide Fragments / immunology
  • Peptide Fragments / toxicity
  • Receptors, CCR4 / antagonists & inhibitors*
  • Receptors, CCR4 / deficiency
  • Receptors, CCR4 / genetics
  • T-Lymphocytes / drug effects
  • T-Lymphocytes / metabolism
  • Time Factors

Substances

  • 2-(3-hydroxy-4,5-diiodo-6H-6-oxoxanthen-9-yl)benzoic acid
  • Ccr4 protein, mouse
  • Cytokines
  • Fluoresceins
  • Myelin-Oligodendrocyte Glycoprotein
  • Peptide Fragments
  • Receptors, CCR4
  • myelin oligodendrocyte glycoprotein (35-55)