Abstract
Changes in the dosage of genes encoding elongation factor EF-1 alpha were shown to cause parallel changes in the misreading of nonsense codons. Higher amounts of EF-1 alpha were correlated with increased nonsense suppression, suggesting that the level of EF-1 alpha is critically involved in translational fidelity.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Cloning, Molecular
-
Gene Amplification / physiology
-
Paromomycin / pharmacology
-
Peptide Elongation Factor 1
-
Peptide Elongation Factors / biosynthesis
-
Peptide Elongation Factors / genetics*
-
Protein Biosynthesis / physiology*
-
Restriction Mapping
-
Saccharomyces cerevisiae / genetics
-
Suppression, Genetic / physiology
Substances
-
Peptide Elongation Factor 1
-
Peptide Elongation Factors
-
Paromomycin