Preparation of Inert Polystyrene Latex Particles as MicroRNA Delivery Vectors by Surfactant-Free RAFT Emulsion Polymerization

Biomacromolecules. 2016 Mar 14;17(3):965-73. doi: 10.1021/acs.biomac.5b01633. Epub 2016 Feb 11.

Abstract

We present the preparation of 11 nm polyacrylamide-stabilized polystyrene latex particles for conjugation to a microRNA model by surfactant-free RAFT emulsion polymerization. Our synthetic strategy involved the preparation of amphiphilic polyacrylamide-block-polystyrene copolymers, which were able to self-assemble into polymeric micelles and "grow" into polystyrene latex particles. The surface of these sterically stabilized particles was postmodified with a disulfide-bearing linker for the attachment of the microRNA model, which can be released from the latex particles under reducing conditions. These nanoparticles offer the advantage of ease of preparation via a scaleable process, and the versatility of their synthesis makes them adaptable to a range of applications.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acrylic Resins / chemistry
  • Drug Carriers / chemical synthesis*
  • Drug Carriers / chemistry
  • Drug Liberation
  • Emulsions / chemistry
  • Latex / chemistry*
  • MicroRNAs / administration & dosage*
  • Nanoparticles / chemistry*
  • Oxidation-Reduction
  • Polymerization
  • Polystyrenes / chemistry*
  • Surface-Active Agents / chemistry

Substances

  • Acrylic Resins
  • Drug Carriers
  • Emulsions
  • Latex
  • MicroRNAs
  • Polystyrenes
  • Surface-Active Agents
  • polyacrylamide