Structural characterization of an acidic Epimedium polysaccharide and its immune-enhancement activity

Abstract

One acidic polysaccharide named EPS-1 was isolated from the aqueous extract of the leaves of Epimedium acuminatum Franch. It may be composed of 1,4-linked α-d-GalpA, 1,3,4-linked α-d-GalpA, 1,6-linked β-d-Galp and terminal α-l-Rhap residues in a molar ratio of 11.0:1.0:1.0:1.0 by chemical and spectroscopic analysis. EPS-1 possessed immune modulation effects on peripheral T lymphocyte and immature chBM-DCs such as promoting the proliferation and cytokine secretion of these cells and increasing the phagocytosis ability of immature chBM-DCs.

Keywords: Epimedium polysaccharide; Immature chBM-DCs; Immune modulation activity; Lymphocyte; Structural characterization; Trifluoroacetic acid (PubChem CID: 6422); d-Fructose (PubChem CID: 5984); d-Galactose (PubChem CID: 6036); d-Galacturonic acid (PubChem CID: 439215); d-Glucose (PubChem CID: 5793); d-Glucuronic acid (PubChem CID: 94715); d-Mannose (PubChem CID: 18950); d-Xylose (PubChem CID: 135191); l-Arabinose (PubChem CID: 439195); l-Rhamnose (PubChem CID: 25310).