Changes in platelet function following cold storage of RBC suspensions

Qian-Li Dang; Jian-Gang Li; Yang Sun; Zhan-Kui Jin; Ying Gao; Cui-Xiang Xu; Ping Chen; Ting Ma; Jiang-Cun Yang

Changes in platelet function following cold storage of RBC suspensions

Int J Clin Exp Med. 2015 Oct 15;8(10):18066-73. eCollection 2015.

Authors

Qian-Li Dang¹, Jian-Gang Li², Yang Sun¹, Zhan-Kui Jin³, Ying Gao⁴, Cui-Xiang Xu⁵, Ping Chen¹, Ting Ma¹, Jiang-Cun Yang¹

Affiliations

¹ Department of Transfusion Medicine, The Third Affiliated Hospital of Medical College of Xi'an Jiaotong University Xi'an 710068, China.
² Department of Transfusion Medicine, Xi'an No. 4 Hospital Xi'an 710004, China.
³ The Second Department of Orthopaedics, The Third Affiliated Hospital of Medical College of Xi'an Jiaotong University Xi'an 710068, China.
⁴ Department of Hematology, The Third Affiliated Hospital of Medical College of Xi'an Jiaotong University Xi'an 710068, China.
⁵ Shaanxi Provincial Center for Clinical Laboratory, The Third Affiliated Hospital of Medical College of Xi'an Jiaotong University Xi'an 710068, China.

PMID: 26770402
PMCID: PMC4694302

Abstract

Objective: To provide a basis for the cold-storage of human platelets as a way to assess changes in platelet function.

Methods: Red blood cell suspensions (11 U and 50 U) were randomly selected at different storage times (3-28 days) and evidence of platelet activation (CD62P) and thromboelastography (TEG) reaction times were investigated.

Results: After 21 days of storage at 4°C, a large number of activated platelets (PAC1+62P+, PAC1-62P+) within the red blood cell suspension (RBCs) retained their function and had TEG-maximum amplitude (TEG-MA) indices in the normal range.

Conclusion: We report that platelets in RBC suspensions retain high activity when stored at 4°C for 21 days. The results provide important information for studies that involve storing platelets under cold conditions.

Keywords: RBC suspensions; blood platelets; cold storage; hemostasis; thromboelastography.