Targeted Next-Generation Sequencing Analysis of a Pendred Syndrome-Associated Thyroid Carcinoma

Endocr Pathol. 2016 Mar;27(1):70-5. doi: 10.1007/s12022-015-9413-4.

Abstract

Pendred syndrome is an autosomal recessive disorder characterized by hearing loss and goiter and is caused by bi-allelic mutations (homozygous or compound heterozygous) of the PDS (SLC26A4) gene. The incidence of Pendred syndrome is 7.5-10/100,000 in the general population, and it carries a 1 % risk of developing thyroid carcinoma. Herein, we report a case of a patient with Pendred syndrome who developed a follicular variant of papillary thyroid carcinoma (FVPTC)-that is approximately at an odd of 1/1,000,000. Targeted next-generation sequencing with ThyroSeq v2 was performed on the tumor, and only a TP53 mutation (TP53 p.R175H) was identified. The mutation was limited to the tumor nodule of FVPTC as shown by immunohistochemistry. This report represents the first extensive molecular study of a Pendred syndrome-associated thyroid carcinoma. The evidences support that thyroid carcinomas arising from dyshormonogenetic goiter require additional genetic alteration in addition to the purported thyroid-stimulating hormone (TSH) overstimulation. It is intrigue to note that the mutant p53 is involved in the development of a low-grade malignant thyroid tumor as FVPTC in this patient.

Keywords: Pendred syndrome; TP53; Thyroid carcinoma.

Publication types

  • Case Reports

MeSH terms

  • Aged
  • Carcinoma / complications*
  • Carcinoma / genetics*
  • Carcinoma, Papillary
  • DNA Mutational Analysis
  • Female
  • Genes, p53
  • Goiter, Nodular / complications*
  • Hearing Loss, Sensorineural / complications*
  • High-Throughput Nucleotide Sequencing
  • Humans
  • Immunohistochemistry
  • Oligonucleotide Array Sequence Analysis
  • Thyroid Cancer, Papillary
  • Thyroid Neoplasms / complications*
  • Thyroid Neoplasms / genetics*
  • Tumor Suppressor Protein p53 / genetics*

Substances

  • TP53 protein, human
  • Tumor Suppressor Protein p53

Supplementary concepts

  • Pendred syndrome