Concentration of Sonication Fluid through Centrifugation Is Superior to Membrane Filtration for Microbial Diagnosis of Orthopedic Implant-Associated Infection

J Clin Microbiol. 2016 Mar;54(3):788-90. doi: 10.1128/JCM.02427-15. Epub 2015 Dec 30.

Abstract

Microbial identification of orthopedic implant-associated infections using sonication fluid (SF) submitted to a concentration step by membrane filtration (SMF) was compared with the standard centrifugation (SC) method. Among 33 retrieved infected implants, sonication identified microorganisms in 26 (78.8%). The sensitivity of SC was higher than that of SMF (78.8% versus 30.3%; P < 0.001).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Centrifugation / methods*
  • Child
  • Female
  • Filtration / methods*
  • Humans
  • Male
  • Microbiological Techniques*
  • Middle Aged
  • Orthopedic Procedures / adverse effects*
  • Prostheses and Implants / adverse effects
  • Prostheses and Implants / microbiology
  • Prosthesis-Related Infections / diagnosis*
  • Prosthesis-Related Infections / microbiology*
  • Young Adult

Grants and funding

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.