Analysis of Assembly and Budding of Lujo Virus

Shuzo Urata; Jacqueline Weyer; Nadia Storm; Yukiko Miyazaki; Petrus Jansen van Vuren; Janusz Tadeusz Paweska; Jiro Yasuda

doi:10.1128/JVI.03198-15

Analysis of Assembly and Budding of Lujo Virus

J Virol. 2015 Dec 30;90(6):3257-61. doi: 10.1128/JVI.03198-15.

Authors

Shuzo Urata¹, Jacqueline Weyer², Nadia Storm², Yukiko Miyazaki³, Petrus Jansen van Vuren², Janusz Tadeusz Paweska², Jiro Yasuda⁴

Affiliations

¹ Department of Emerging Infectious Diseases, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan.
² Center for Emerging and Zoonotic Diseases, National Institute for Communicable Diseases (NICD), Division of the National Health Laboratory Service, Sandringham, South Africa.
³ Department of Emerging Infectious Diseases, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan The Graduate School of Biomedical Science, Nagasaki University, Nagasaki, Japan.
⁴ Department of Emerging Infectious Diseases, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan The Graduate School of Biomedical Science, Nagasaki University, Nagasaki, Japan j-yasuda@nagasaki-u.ac.jp.

Abstract

The recently identified arenavirus Lujo virus (LUJV) causes fatal hemorrhagic fever in humans. We analyzed its mechanism of viral release driven by matrix protein Z and the cell surface glycoprotein precursor GPC. The L domains in Z are required for efficient virus-like particle release, but Tsg101, ALIX/AIP1, and Vps4A/B are unnecessary for budding. LUJV GPC is cleaved by site 1 protease (S1P) at the RKLM motif, and treatment with the S1P inhibitor PF-429242 reduced LUJV production.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Humans
Lujo virus / growth & development
Lujo virus / physiology*
Viral Proteins / metabolism
Virus Assembly*
Virus Release*

Substances

Viral Proteins