Evaluation of a multiplex OnSpot Primer-Extension PCR assay in the diagnosis of sepsis

Ludwig Knabl; Wolfgang Mutschlechner; Dorothea Orth-Höller

doi:10.1016/j.mimet.2015.12.001

Evaluation of a multiplex OnSpot Primer-Extension PCR assay in the diagnosis of sepsis

J Microbiol Methods. 2016 Jan:120:91-3. doi: 10.1016/j.mimet.2015.12.001. Epub 2015 Dec 2.

Authors

Ludwig Knabl¹, Wolfgang Mutschlechner², Dorothea Orth-Höller³

Affiliations

¹ Division of Hygiene and Medical Microbiology, Medical University Innsbruck, Schöpfstrasse 41, 6020 Innsbruck, Austria. Electronic address: ludwig.knabl@i-med.ac.at.
² Division of Hygiene and Medical Microbiology, Medical University Innsbruck, Schöpfstrasse 41, 6020 Innsbruck, Austria. Electronic address: wolfgang.mutschlechner@i-med.ac.at.
³ Division of Hygiene and Medical Microbiology, Medical University Innsbruck, Schöpfstrasse 41, 6020 Innsbruck, Austria.

PMID: 26658328
DOI: 10.1016/j.mimet.2015.12.001

Abstract

For evaluation of the Anagnostics Pathogens DNA xA (PxA) assay in the diagnosis of sepsis 58 blood specimens were tested in comparison with the LightCycler SeptiFast assay and blood culture as gold standard. The PxA assay had a lower sensitivity (0.63 vs. 0.8), but higher specificity (0.83 vs. 0.67) than SeptiFast.

Keywords: Hybcell; LightCycler SeptiFast; Microarray assay; Multiplex PCR assay; Sepsis diagnostic.

MeSH terms

Bacteremia / blood
Bacteremia / diagnosis
Bacteremia / microbiology
Blood-Borne Pathogens / isolation & purification
DNA, Bacterial / blood
DNA, Fungal / blood
Female
Fungemia / blood
Fungemia / diagnosis
Fungemia / microbiology
Humans
Male
Middle Aged
Multiplex Polymerase Chain Reaction / methods*
Pilot Projects
Real-Time Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Sepsis / blood
Sepsis / diagnosis*
Sepsis / microbiology

Substances

DNA, Bacterial
DNA, Fungal