Biomarkers of oxidative-stress and inflammation in exhaled breath condensate from hospital cleaners

Biomarkers. 2016;21(2):115-22. doi: 10.3109/1354750X.2015.1118541. Epub 2015 Dec 9.

Abstract

Objective: We studied the impact of chlorinated agents exposure on exhaled breath condensate (EBC) biomarkers in cleaners.

Methods: Malondialdehyde (MDA), 4-hydroxynonenal (4-HNE), nitrites (NO2(-)), nitrates (NO3(-)), pH, hydrogen peroxide (H2O2) and ammonium (NH3(+)) were tested in EBC of 40 cleaners and 40 non-exposed controls. Pentraxin-3 (PTX3) and soluble type II receptor of IL-1 (sIL-1RII) were analyzed also in plasma.

Results: Levels of MDA-EBC, 4-HNE-EBC and NO3(-)-EBC were higher, while pH-EBC values were lower, in cleaners. MDA-EBC was associated with 4-HNE-EBC, NO3(-)-EBC and pH. 4-HNE-EBC correlated with PTX3.

Conclusion: Professional exposure to chlorinated agents increases EBC biomarkers of oxidative stress and inflammation.

Keywords: Biological effect markers; Pentraxin-3; cleaners; exhaled breath condensate; soluble interleukin-1 receptor type II.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aldehydes / metabolism
  • Ammonium Compounds / metabolism
  • Biomarkers / blood
  • Biomarkers / metabolism*
  • Breath Tests
  • C-Reactive Protein / metabolism
  • Cross-Sectional Studies
  • Enzyme-Linked Immunosorbent Assay
  • Exhalation
  • Housekeeping, Hospital*
  • Humans
  • Hydrogen Peroxide / metabolism
  • Hydrogen-Ion Concentration
  • Inflammation / blood
  • Inflammation / metabolism*
  • Linear Models
  • Malondialdehyde / metabolism
  • Middle Aged
  • Nitrates / metabolism
  • Nitrites / metabolism
  • Occupational Diseases / blood
  • Occupational Diseases / diagnosis
  • Occupational Diseases / metabolism*
  • Occupational Exposure / analysis
  • Oxidative Stress*
  • Receptors, Interleukin-1 Type I / blood
  • Serum Amyloid P-Component / metabolism
  • Surveys and Questionnaires
  • Tandem Mass Spectrometry

Substances

  • Aldehydes
  • Ammonium Compounds
  • Biomarkers
  • Nitrates
  • Nitrites
  • Receptors, Interleukin-1 Type I
  • Serum Amyloid P-Component
  • PTX3 protein
  • Malondialdehyde
  • C-Reactive Protein
  • Hydrogen Peroxide
  • 4-hydroxy-2-nonenal