A tool for the selective sequestration of ATP and PPi to aid in-solution phosphopeptide detection assays

Analyst. 2016 Feb 7;141(3):820-2. doi: 10.1039/c5an01414j. Epub 2015 Dec 8.

Abstract

The presence of small phospho-anions, such as PPi and ATP in protein samples often complicates the robust detection of phosphoproteins by metal-based chemosensors and receptors. We herein report the development of a bis(Zn(2+)-cyclen)-triethylbenzene scaffold which can selectively sequester PPi and ATP without affecting the detection of a di-phosphorylated peptide by a ProxyPhos chemosensor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / chemistry*
  • Benzene Derivatives / chemistry
  • Coordination Complexes / chemistry*
  • Diphosphates / chemistry*
  • Macrocyclic Compounds / chemistry
  • Phosphopeptides / analysis*
  • Zinc / chemistry

Substances

  • Benzene Derivatives
  • Coordination Complexes
  • Diphosphates
  • Macrocyclic Compounds
  • Phosphopeptides
  • diphosphoric acid
  • Adenosine Triphosphate
  • Zinc