Preparation of Escherichia coli elongation factor Tu-guanosine 5'-triphosphate analogs

Anal Biochem. 1989 Feb 15;177(1):188-93. doi: 10.1016/0003-2697(89)90038-9.

Abstract

A simple procedure for the bulk preparation of 20 mg of Escherichia coli elongation factor (EF)-Tu-GTP analogs is described. The protocol is based upon the preparation and stabilization of nucleotide-free EF-Tu using an EF-Ts affinity chromatographic resin. The procedure is a general one for the preparation of any GTP analog of EF-Tu.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Binding, Competitive
  • Chromatography, Affinity
  • Escherichia coli
  • Guanine Nucleotides / isolation & purification
  • Guanosine Triphosphate / analogs & derivatives*
  • Guanosine Triphosphate / chemical synthesis
  • Peptide Elongation Factor Tu / analogs & derivatives*
  • Peptide Elongation Factor Tu / chemical synthesis
  • Peptide Elongation Factors
  • Resins, Plant
  • Sepharose

Substances

  • Guanine Nucleotides
  • Peptide Elongation Factors
  • Resins, Plant
  • elongation factor Ts
  • Guanosine Triphosphate
  • Sepharose
  • Peptide Elongation Factor Tu