Performance of the Cryptococcal Antigen Lateral Flow Assay in Non-HIV-Related Cryptococcosis

Anupop Jitmuang; Anil A Panackal; Peter R Williamson; John E Bennett; John P Dekker; Adrian M Zelazny

doi:10.1128/JCM.02223-15

Performance of the Cryptococcal Antigen Lateral Flow Assay in Non-HIV-Related Cryptococcosis

J Clin Microbiol. 2016 Feb;54(2):460-3. doi: 10.1128/JCM.02223-15. Epub 2015 Nov 25.

Authors

Anupop Jitmuang¹, Anil A Panackal², Peter R Williamson², John E Bennett², John P Dekker³, Adrian M Zelazny⁴

Affiliations

¹ Microbiology Service, Department of Laboratory Medicine, National Institutes of Health, Bethesda, Maryland, USA Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
² National Institute of Allergy and Infectious Diseases, Clinical Center, National Institutes of Health, Bethesda, Maryland, USA.
³ Microbiology Service, Department of Laboratory Medicine, National Institutes of Health, Bethesda, Maryland, USA.
⁴ Microbiology Service, Department of Laboratory Medicine, National Institutes of Health, Bethesda, Maryland, USA azelazny@cc.nih.gov.

Abstract

The cryptococcal antigen lateral flow assay (CrAg LFA) was evaluated for the diagnosis of cryptococcosis in HIV-negative patients. The sensitivity was excellent, suggesting that this assay can replace conventional testing based on latex agglutination (LA). CrAg LFA and LA titers were correlated but were not directly comparable, with implications for conversion between assays.

Publication types

Research Support, N.I.H., Intramural

MeSH terms

Adult
Antigens, Fungal / immunology*
Cryptococcosis / diagnosis*
Cryptococcosis / microbiology*
Cryptococcus / immunology*
Female
Humans
Immunologic Tests* / methods
Immunologic Tests* / standards
Male
Middle Aged
Reproducibility of Results
Sensitivity and Specificity

Substances

Antigens, Fungal

Grants and funding

Intramural NIH HHS/United States