FRET-Based Probe for Monitoring pH Changes in Lipid-Dense Region of Hct116 Cells

Upendar Reddy G; Anila H A; Firoj Ali; Nandaraj Taye; Samit Chattopadhyay; Amitava Das

doi:10.1021/acs.orglett.5b02568

FRET-Based Probe for Monitoring pH Changes in Lipid-Dense Region of Hct116 Cells

Org Lett. 2015 Nov 20;17(22):5532-5. doi: 10.1021/acs.orglett.5b02568. Epub 2015 Nov 2.

Authors

Upendar Reddy G¹, Anila H A¹, Firoj Ali¹, Nandaraj Taye², Samit Chattopadhyay², Amitava Das¹

Affiliations

¹ Organic Chemistry Division, CSIR-National Chemical Laboratory , Pune 411008, India.
² Chromatin and Disease Biology Laboratory, National Centre for Cell Science , Pune 411007, India.

PMID: 26555683
DOI: 10.1021/acs.orglett.5b02568

Abstract

A rhodamine conjugate (L) with a pseudo Stokes shift of 165 nm is used for probing changes in solution pH under physiological conditions. This reagent is found to be nontoxic, and the luminescence response could be used for imaging changes in endogenous pH induced by dexamethanose (DMT) in the endoplasmic reticulum.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Endoplasmic Reticulum / drug effects
Fluorescent Dyes / chemistry*
HCT116 Cells
Humans
Hydrogen-Ion Concentration
Lipids / analysis*
Luminescence
Molecular Structure
Rhodamines / chemistry

Substances

Fluorescent Dyes
Lipids
Rhodamines