Up-regulation of CYLD enhances Listeria monocytogenes induced apoptosis in THP-1 cells

Microb Pathog. 2016 Jan:90:50-4. doi: 10.1016/j.micpath.2015.10.007. Epub 2015 Nov 3.

Abstract

Listeria monocytogenes (Lm), a facultative anaerobic gram-positive bacterium, causes listeriosis. Immune cell apoptosis is considered to be one pathogenic factor for listeriosis. As a deubiquitinase, CYLD is an important regulator both in innate immune response and apoptosis by negatively modulating NF-κB pathway. However the role of CYLD in Lm induced apoptosis remains unclear. Here we found that CYLD is significantly up-regulated in macrophages upon its infection. There is a moderate decrease in Lm proliferation and apoptotic cells in siRNA-induced CYLD knockdown THP-1 cells. Thereby CYLD may be involved in cell apoptosis mediated by Lm infection and its proliferation.

Keywords: Apoptosis; CYLD; Listeria monocytogenes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / immunology
  • Cell Line
  • Deubiquitinating Enzyme CYLD
  • Flow Cytometry
  • Gene Knockdown Techniques
  • Humans
  • Immunity, Innate
  • Listeria monocytogenes / immunology*
  • Listeria monocytogenes / metabolism*
  • Listeria monocytogenes / pathogenicity
  • Listeriosis / immunology*
  • Listeriosis / metabolism
  • Listeriosis / microbiology
  • Macrophages / immunology*
  • Macrophages / metabolism
  • Macrophages / microbiology*
  • NF-kappa B / immunology
  • NF-kappa B / metabolism
  • RNA, Small Interfering / administration & dosage
  • RNA, Small Interfering / genetics
  • Transcriptional Activation
  • Tumor Suppressor Proteins / genetics
  • Tumor Suppressor Proteins / immunology*
  • Tumor Suppressor Proteins / metabolism
  • Up-Regulation / immunology

Substances

  • NF-kappa B
  • RNA, Small Interfering
  • Tumor Suppressor Proteins
  • CYLD protein, human
  • Deubiquitinating Enzyme CYLD